Characterization, functional analysis and application of 4-Coumarate: CoA ligase genes from Populus trichocarpa.
Identifieur interne : 000A82 ( Main/Exploration ); précédent : 000A81; suivant : 000A83Characterization, functional analysis and application of 4-Coumarate: CoA ligase genes from Populus trichocarpa.
Auteurs : Chen Zhang [République populaire de Chine] ; Ying Zang [République populaire de Chine] ; Peng Liu [République populaire de Chine] ; Zhaojuan Zheng [République populaire de Chine] ; Jia Ouyang [République populaire de Chine]Source :
- Journal of biotechnology [ 1873-4863 ] ; 2019.
Descripteurs français
- KwdFr :
- MESH :
- enzymologie : Populus.
- métabolisme : Coenzyme A ligases, Propanols, Propionates.
- Cinétique.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Coenzyme A Ligases, Propanols, Propionates.
- enzymology : Populus.
- Kinetics.
Abstract
4-Coumarate: CoA ligase (4CL) is an important branch point directing metabolites to flavonoid or monolignol pathways in plants. It plays a vital role in the biosynthesis of plant nature products in microbes. Herein, Ptr4CL4, Ptr4CL5 and Ptr4CL7 from Populus trichocarpa were cloned and expressed in Escherichia coli. Two recombinant proteins Ptr4CL4 and Ptr4CL5 showed distinct activities for different substrates. The Ptr4CL4, not previously reported, showed the highest affinity and activity for p-coumaric acid, but a unique substrate self-inhibition was observed at high concentration of p-coumaric acid. Ptr4CL5 was suitable for pathway construction due to no self-substrate inhibition and high initial reaction rate. To explore the potential of Ptr4CL5 in biosynthesis of cinnamyl alcohol, a biosynthesis pathway established with Ptr4CL5, PtrCCR2, endogenous reductases was constructed in E. coli and the titer of cinnamyl alcohol reached 4.8 mM which is higher than other reports. The result indicates that the wood-derived Ptr4CL5 has signification potential in the biosynthesis of cinnamyl alcohol and other monolignol derivatives.
DOI: 10.1016/j.jbiotec.2019.06.300
PubMed: 31233773
Affiliations:
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It plays a vital role in the biosynthesis of plant nature products in microbes. Herein, Ptr4CL4, Ptr4CL5 and Ptr4CL7 from Populus trichocarpa were cloned and expressed in Escherichia coli. Two recombinant proteins Ptr4CL4 and Ptr4CL5 showed distinct activities for different substrates. The Ptr4CL4, not previously reported, showed the highest affinity and activity for p-coumaric acid, but a unique substrate self-inhibition was observed at high concentration of p-coumaric acid. Ptr4CL5 was suitable for pathway construction due to no self-substrate inhibition and high initial reaction rate. To explore the potential of Ptr4CL5 in biosynthesis of cinnamyl alcohol, a biosynthesis pathway established with Ptr4CL5, PtrCCR2, endogenous reductases was constructed in E. coli and the titer of cinnamyl alcohol reached 4.8 mM which is higher than other reports. The result indicates that the wood-derived Ptr4CL5 has signification potential in the biosynthesis of cinnamyl alcohol and other monolignol derivatives.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31233773</PMID><DateCompleted><Year>2019</Year><Month>12</Month><Day>30</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-4863</ISSN><JournalIssue CitedMedium="Internet"><Volume>302</Volume><PubDate><Year>2019</Year><Month>Aug</Month><Day>20</Day></PubDate></JournalIssue><Title>Journal of biotechnology</Title><ISOAbbreviation>J Biotechnol</ISOAbbreviation></Journal><ArticleTitle>Characterization, functional analysis and application of 4-Coumarate: CoA ligase genes from Populus trichocarpa.</ArticleTitle><Pagination><MedlinePgn>92-100</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0168-1656(19)30779-5</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.jbiotec.2019.06.300</ELocationID><Abstract><AbstractText>4-Coumarate: CoA ligase (4CL) is an important branch point directing metabolites to flavonoid or monolignol pathways in plants. It plays a vital role in the biosynthesis of plant nature products in microbes. Herein, Ptr4CL4, Ptr4CL5 and Ptr4CL7 from Populus trichocarpa were cloned and expressed in Escherichia coli. Two recombinant proteins Ptr4CL4 and Ptr4CL5 showed distinct activities for different substrates. The Ptr4CL4, not previously reported, showed the highest affinity and activity for p-coumaric acid, but a unique substrate self-inhibition was observed at high concentration of p-coumaric acid. Ptr4CL5 was suitable for pathway construction due to no self-substrate inhibition and high initial reaction rate. To explore the potential of Ptr4CL5 in biosynthesis of cinnamyl alcohol, a biosynthesis pathway established with Ptr4CL5, PtrCCR2, endogenous reductases was constructed in E. coli and the titer of cinnamyl alcohol reached 4.8 mM which is higher than other reports. The result indicates that the wood-derived Ptr4CL5 has signification potential in the biosynthesis of cinnamyl alcohol and other monolignol derivatives.</AbstractText><CopyrightInformation>Copyright © 2019 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Chen</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, 210037, PR China; College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zang</LastName><ForeName>Ying</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>College of Forestry, Nanjing Forestry University, Nanjing, 210037, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Peng</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>College of Forestry, Nanjing Forestry University, Nanjing, 210037, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zheng</LastName><ForeName>Zhaojuan</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, 210037, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ouyang</LastName><ForeName>Jia</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, 210037, PR China; College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, PR China. Electronic address: hgouyj@njfu.edu.cn.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>06</Month><Day>21</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>J Biotechnol</MedlineTA><NlmUniqueID>8411927</NlmUniqueID><ISSNLinking>0168-1656</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D020005">Propanols</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011422">Propionates</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 6.2.1.-</RegistryNumber><NameOfSubstance UI="D003066">Coenzyme A Ligases</NameOfSubstance></Chemical><Chemical><RegistryNumber>IBS9D1EU3J</RegistryNumber><NameOfSubstance UI="C495469">trans-3-(4'-hydroxyphenyl)-2-propenoic acid</NameOfSubstance></Chemical><Chemical><RegistryNumber>SS8YOP444F</RegistryNumber><NameOfSubstance UI="C020722">cinnamyl alcohol</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D003066" MajorTopicYN="N">Coenzyme A Ligases</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007700" MajorTopicYN="N">Kinetics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020005" MajorTopicYN="N">Propanols</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011422" MajorTopicYN="N">Propionates</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">4-Coumarate: CoA ligase</Keyword><Keyword MajorTopicYN="N">Cinnamyl alcohol</Keyword><Keyword MajorTopicYN="N">Kinetic characteristic</Keyword><Keyword MajorTopicYN="N">Populus trichocarpa</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>04</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2019</Year><Month>05</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>06</Month><Day>21</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>6</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>12</Month><Day>31</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>6</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31233773</ArticleId><ArticleId IdType="pii">S0168-1656(19)30779-5</ArticleId><ArticleId IdType="doi">10.1016/j.jbiotec.2019.06.300</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Zhang, Chen" sort="Zhang, Chen" uniqKey="Zhang C" first="Chen" last="Zhang">Chen Zhang</name></noRegion><name sortKey="Liu, Peng" sort="Liu, Peng" uniqKey="Liu P" first="Peng" last="Liu">Peng Liu</name><name sortKey="Ouyang, Jia" sort="Ouyang, Jia" uniqKey="Ouyang J" first="Jia" last="Ouyang">Jia Ouyang</name><name sortKey="Zang, Ying" sort="Zang, Ying" uniqKey="Zang Y" first="Ying" last="Zang">Ying Zang</name><name sortKey="Zheng, Zhaojuan" sort="Zheng, Zhaojuan" uniqKey="Zheng Z" first="Zhaojuan" last="Zheng">Zhaojuan Zheng</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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